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Document Type |
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Thesis |
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Document Title |
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Antimicrobial Activity of The Extract of The Plant Cymbopogon schoenanthus دراسة تأثير مستخلص نبات الإذخر على نشاط بعض الميكروبات الممرضة |
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Subject |
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Medicinal plants |
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Document Language |
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English |
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Abstract |
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Cymbopogon schoenanthus is a wild plant that grows in parts of the Arabian Peninsula and other regions of the world. It is useful in treating kidney stones and many infections. The primary interest of the study was to investigate this claim as to prove it in vitro so that it can have scientific validation. C. schoenanthus was collected, identified and its essential oil was extracted. Inhibitory effects of the extract were detected using agar-well diffusion test on five pathogens of Gram-positive bacteria; Staphylococcus aureus, methicillin-resistant S. aureus (MRSA), S. saprophyticus and two Gram-negative bacteria; Escherichia coli and Klebsiella pneumoniae. The broth microdilution test was used to determine the minimum inhibitory concentrations. The oil showed potent activity towards E. coli (MIC: 3.125 mg/ml), S. aureus (MIC: 1.56 mg/ml), MRSA (MIC: 0.781 mg/ml) and K. pneumoniae (MIC: 0.781 mg/ml). Time–kill curves were obtained using the minimum inhibitory concentration (MIC) of the indicated essential oil to concentrations of 1.563, 0.781 and 0.781 mg/ml for testing S. aureus, MRSA and K. pneumoniae respectively. Within 2 hours of incubation, 99.99% of K. pneumoniae were killed; the same for S. saprophyticus and for E. coli 99.99% were killed within 4 hours. For S. aureus, approximately 96.3% of cells were killed within 24 hours and for MRSA, 78.3 % of cells were killed in 24 hours. Subsequently, the essential oil of C. schoenanthus was subjected to chemical analysis by GC/MS. The eight major components were identified as piperitone (14.6%), cyclohexane methanol (11.6%), β-elemene (11.6%), α-eudesmol (11.5%), elemol (10.8%), β-eudesmol (8.5%), 2-Naphthalenemethanol (7.1%) and γ-eudesmol (4.2%). Toxicity study using the brine shrimp lethality test showed no toxicity for the all tested concentrations. Anti-tumor activity was recorded for Breast carcinoma HTB-26 and Colon cancer HTB-38 at LC50=100 and 75 μg/ml respectively but no activity against Ehrlich carcinoma. |
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Supervisor |
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Saleh A. Kabli |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1434 AH
2013 AD |
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Number Of Pages |
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117 |
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Co-Supervisor |
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Dr. Seif-Eldin N. Ayyad |
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Added Date |
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Wednesday, September 18, 2013 |
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Researchers
| غزل محمد هاشم | Hashim, Ghazal Mohamed | Investigator | Master | |
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