Document Details
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Document Type |
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Thesis |
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Document Title |
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FTIR Spectroscopy Studying the Cytotoxic Effect of 2, 4-Dichlorophenoxyacetic Acid on Rat Tissues and the Antitoxic Properties of Lepidium sativum Using دراسة التأثير السمي للمبيد 2, 4-داي كلورو فينوكسي أسيتيك أسيد على أنسجة الجرذان والتأثير مضاد السمية لنبات حب الرشاد باستخدام تفنية مطياف الأشعة تحت الحمراء |
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Subject |
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Dichlorophenoxyacetic acid--Toxicology |
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Document Language |
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English |
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Abstract |
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2, 4-Dichlorophenoxyacetic acid (2, 4-D) is a herbicide used in agriculture and it is known to cause apoptosis in animals and humans. Fourier transform infrared (FTIR) spectroscopy appears to be a valuable technique used to investigate peroxidation induced in living cells. In this study, the neurotoxic and hepatotoxic effects of 2, 4-D as well as the antitoxic-antioxidant properties of Lepidium sativum (LS) water extract on rat cerebellum and liver tissues were investigated by FTIR spectroscopy and eletron microscopy (EM). Twenty five albino Wistar rats were grouped into 2, 4-D LD50, curative, protective and LS water extract treated group. A significant reduction in protein content and marked changes in the secondary structure of protein were detected after 2,4-D intoxication. Looseness of membrane lipid chain packing, lipid polarity and/or a significant increase in the formation of lipids hydroperoxyl groups and carbonyl compounds have been shown in the 2, 4-D LD50 treated group as well as the water control group. For the LS treated groups, a remarkable variations in the above mentioned results were observed. The results revealed that the curative and LS only treated cerebellum tissue had overcome toxicity and age-related damage compared to the water control group. While for the protective group, this effect was not as significant as the LS only and curative groups. Glycogen is dramatically decreased in rat liver after the induction of 2, 4-D, while curative and LS only treated groups showed a remarkable recovery in the glycogen band areas. Unexpectedly, the protective group showed a massive depletion in the IR glycogen bands. To conclude, this study provides evidence that 2,4-D directly interacts with membranes influencing lipid polarity, looseness, hydrocarbon chain length and fluidity as well as protein content and conformational structure. It is also established that the LS water extract has a significant potency as an antitoxic/antioxidant effect against hepatotoxicity and neurotoxity induced by 2,4-D. The EM and FTIR results also demonstrated that 2,4-D toxicity causes cell death by apoptosis. FT-IR spectroscopy proved to be a rapid and sensitive monitoring of toxins-induced damage in biological membranes and proteins. |
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Supervisor |
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Gehan Abdel Raouf |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1433 AH
2012 AD |
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Number Of Pages |
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134 |
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Co-Supervisor |
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Safa’a Yousef Quisty |
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Added Date |
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Tuesday, November 5, 2013 |
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Researchers
| تهاني حسين دخاخني | Dakhakhni, Thani Hussein | Investigator | Master | |
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